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rabbit monoclonal anti psmad2 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc rabbit monoclonal anti psmad2
Rabbit Monoclonal Anti Psmad2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+psmad2/pm41845215-88-32-59
Average 86 stars, based on 1 article reviews

rabbit monoclonal anti psmad2 - by Bioz Stars, 2026-09

86/100 stars

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Article Title: Dissecting TGF-β-induced glioblastoma invasion with engineered hyaluronic acid hydrogels
Article Snippet: Membranes were probed with rabbit anti-SMAD2 antibody (Cell Signaling Technology, Catalog No. 5339) or rabbit anti-pSMAD2 (Cell Signaling Technology, catalog no. 18338T), and mouse anti-β-actin (Sigma-Aldrich, Catalog No. A2228).

Article Title: Chromatin Remodelling in Damaged Intestinal Crypts Orchestrates Redundant TGFβ and Hippo Signalling to Drive Regeneration
Article Snippet: The following primary antibodies were used: rabbit anti-lysozyme (Dako, #2230, 1:1,000), rabbit anti-Muc2 (Santa Cruz, #sc-15334, 1:300), rabbit anti-Olfm4 (Cell Signaling, #66479, 1:300), rabbit anti-ChgA (Abcam, #ab85554, 1:250), goat anti-GFP (Abcam, #ab5450, 1:1000), chicken anti-GFP (Abcam, #ab13970; 1:1000) Rabbit anti-ki67(Abcam, #ab15580), Anti-CD326 (Biolegend, #118205), Rabbit anti-Smad2/3 (Cell Signaling, #8685S, 1:300), Rabbit anti-pSmad2 (Cell signaling, #18338, 1:200), Rabbit anti-Smad7 (Abcam, #ab216428; 1:100).

Article Title: GGTLC1 knockdown inhibits the progression of endometrial cancer by regulating the TGF-β/Smad signaling pathway
Article Snippet: The primary antibodies that were employed are as follows: rabbit anti-GGTLC1 (1:1000, Signalway Antibody, cat. no. 40020), rabbit anti-pSmad2 (1:1000, Cell Signaling Technology, cat. no. 3108S), rabbit anti-Smad2 (1:1000, Cell Signaling Technology, cat. no. 5339S), rabbit anti-pSmad3 (1:2000, Boster Biological Technology, cat. no. BM4033), rabbit anti-Smad3 (1:2000, Boster Biological Technology, cat. no. BM3919), rabbit anti-TGF-β1/2 (1:1000, Beyotime Biotechnology, cat. no. AF0297), rabbit anti-caspase3 (1:1000, HuaBio, China, cat. no. ET1602-39), rabbit anti-bax (1:5000, HuaBio, China, cat. no. ET1603-34), rabbit anti-p21 (1:1000, Wanlei, China, cat. no. WL0362), rabbit anti-bcl2 (1:1000, HuaBio, China, cat. no. ET1702-53).

Article Title: Temporal- and concentration-dependent effects of transforming growth factor-beta 1 (TGF-β1) in primary human knee fibroblasts
Article Snippet: The following antibodies were used: Rabbit anti-pSMAD2 (Ser465/467, 1:1,000, #3108, Cell Signaling Technology, USA), rabbit anti-SMAD2 (1:1,000, #5339, Cell Signaling Technology), mouse anti-α-SMA (1:3,000, ab7817, Abcam, UK), rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:30,000, ab181602, Abcam), goat anti-rabbit secondary (1:5,000, ab205718, Abcam), and goat anti-mouse secondary (1:5,000, ab205719, Abcam).

Membrane:

Article Title: Fibrillin-1 G234D mutation in the hybrid1 domain causes tight skin associated with dysregulated elastogenesis and increased collagen cross-linking in mice.
Article Snippet: The lysates were mixed with 3 x SDS sample buffer with 2-mercaptoethanol (Wako, 133–14371) and boiled at 95 ◦C for 5 min, and then were subjected to SDS-PAGE. .. Proteins were transferred to a PVDF membrane (Millipore, IPVH00010) and immunoblotted with primary antibodies as follows: mouse anti-fibronectin (1:1000, BD Transduction Laboratories, 610078), rabbit anti-Loxl1 (1:1000, Abcam, ab313585), rabbit anti-pSmad2 (1:500, Cell Signaling, 31084S), rabbit anti-Smad2/3 (1:3000, Cell Signaling, 8685S), rabbit anti-pSmad1/5 (1:1000, Cell Signaling, 9516S), rabbit anti-fibrillin-1 (1:1000, rf18, a kind gift from Tomoyuki Nakamura), mouse anti-collagen-1 (1:1000, Millipore, AB765P) and rabbit anti-GAPDH (1:1000, Cell Signaling, 2118). .. Membranes were then incubated with respective anti-rabbit or -mouse HRP-conjugated secondary antibody (1:1000, Bio-Rad) and visualized with a chemoluminescence kit (Ez WestLumiplus, Atto Corporation, WSE 7120S HRP).

Cell-Signaling:

Article Title: Linking expression and function of Drosophila type-I TGF-β receptor baboon isoforms: Multiple roles of BaboA isoform in shaping of the adult central nervous system
Article Snippet: The membranes were then blocked with Casein-containing buffer (Bio-Rad, #1610783) and incubated with primary antibodies at 4°C overnight. .. The primary antibodies were rabbit anti-pSmad2 (1:500, Cell signaling Technology, #3108) and anti-β-Tubulin (1:1000, DSHB, E7). .. The immunoreactive bands were detected using HRP-conjugated secondary antibodies (anti-rabbit and anti-mouse IgGs, 1:10,000, Cell signaling Technology), developed using Pierce ECL Western Blotting Substrate (Thermo Scientific, #32209), and chemiluminescence detected after exposure to X-ray film (Genesee Scientific).

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A. Schematic showing two possible mechanisms to spatially pattern proliferation in the embryonic lung. B. Representative immunoblots for pSMAD2 and GAPDH in lungs cultured with RepSox or vehicle control, and graph showing the normalized pSMAD2 band intensity from 3 replicates. Shown are the average and s.d. of normalized intensity of the pSMAD2 band. C. Fluorescence images of EdU incorporation in the epithelium and mesenchyme of lung explants cultured with RepSox or vehicle control. White arrows denote closely apposed branch stalks. Solid and dotted boxes denote the regions between b1 and b2, respectively. Scale bars, 100 µm. D. Graph showing the percentage of EdU-positive epithelial cells in lung explants cultured with RepSox or vehicle control. Shown are the average and s.d. of 6 lungs per condition. n.s. stands for not significant. *** p <0.001. E. Graph showing the percentage of EdU-positive mesenchymal cells in lung explants cultured with RepSox or vehicle control. Shown are the average and s.d. of 3 lungs per condition. ** p <0.005. F. Graph showing the density of mesenchymal cells near the tip in lung explants cultured with RepSox or vehicle control. Shown are the average and s.d. of at least 4 lungs per condition. * p <0.05.

Journal: bioRxiv

Article Title: TGFβ determines epithelial tissue spacing by regulating mesenchymal condensation

doi: 10.64898/2026.03.16.712215

Figure Lengend Snippet: A. Schematic showing two possible mechanisms to spatially pattern proliferation in the embryonic lung. B. Representative immunoblots for pSMAD2 and GAPDH in lungs cultured with RepSox or vehicle control, and graph showing the normalized pSMAD2 band intensity from 3 replicates. Shown are the average and s.d. of normalized intensity of the pSMAD2 band. C. Fluorescence images of EdU incorporation in the epithelium and mesenchyme of lung explants cultured with RepSox or vehicle control. White arrows denote closely apposed branch stalks. Solid and dotted boxes denote the regions between b1 and b2, respectively. Scale bars, 100 µm. D. Graph showing the percentage of EdU-positive epithelial cells in lung explants cultured with RepSox or vehicle control. Shown are the average and s.d. of 6 lungs per condition. n.s. stands for not significant. *** p <0.001. E. Graph showing the percentage of EdU-positive mesenchymal cells in lung explants cultured with RepSox or vehicle control. Shown are the average and s.d. of 3 lungs per condition. ** p <0.005. F. Graph showing the density of mesenchymal cells near the tip in lung explants cultured with RepSox or vehicle control. Shown are the average and s.d. of at least 4 lungs per condition. * p <0.05.

Article Snippet: Samples were then incubated with primary antibodies against E-cadherin (1:100; BD Biosciences, 610182), pSMAD1/5/9 (1:100; Invitrogen, PA5-64711), pan-laminin (1:100; Invitrogen, PA1-16730), perlecan (1:100; Invitrogen, MA1-06821), or pSMAD2 (1:100; Cell Signaling, 18338).

Techniques: Western Blot, Cell Culture, Control, Fluorescence

( A ) Fluorescence images of nuclei (blue) and EdU incorporation (red) in lung explants inserted with TGFβ-containing bead or vehicle control. The beads were inserted proximal to b1. In contrast to control, few EdU-positive mesenchymal cells are observed in TGFβ-containing bead-inserted lung. White arrows denote proximal stalk of b1. ( B ) Fluorescence images of epithelium in branch-implanted lung explants treated with RepSox or vehicle control. The distance between implanted and native branches is small in RepSox-treated lungs. Scale bars, 100 µm and 10 µm (inset). ( C ) Fluorescence images of nuclei in lung explants cultured for 12- or 24-hours after implantation of beads with different TGFβ concentrations. ( D ) Graph showing the sizes of condensations in bead-implanted lung explants with different initial TGFβ concentration and culture time. ( E ) Fluorescence images of nuclei (blue) and pSMAD2 (red) counterstained for E-cadherin (green). Strong circular pSMAD2 staining is observed near TGFβ-containing beads. ( F ) Fluorescence images of nuclei (blue) and perlecan (red) in lung explants inserted with TGFβ-containing bead or vehicle control. Strong perlecan staining is observed in mesenchymal condensations near TGFβ-containing beads. ( G ) Fluorescence images of nuclei in lung explants cultured with or without RepSox after being inserted with TGFβ-containing beads or vehicle control. Mesenchymal condensations are only observed in lungs inserted with TGFβ-containing beads and cultured without RepSox. ( H ) pSMAD2 staining and nuclear-masked pSMAD2 staining intensity near the branch. Scale bar, 50 µm. Subepithelial mesenchymal cells exhibit strong pSMAD2 staining. Cyan dotted lines denote condensations ( D, F, G ). Scale bars, 100 µm ( A, C, E, F, G ).

Journal: bioRxiv

Article Title: TGFβ determines epithelial tissue spacing by regulating mesenchymal condensation

doi: 10.64898/2026.03.16.712215

Figure Lengend Snippet: ( A ) Fluorescence images of nuclei (blue) and EdU incorporation (red) in lung explants inserted with TGFβ-containing bead or vehicle control. The beads were inserted proximal to b1. In contrast to control, few EdU-positive mesenchymal cells are observed in TGFβ-containing bead-inserted lung. White arrows denote proximal stalk of b1. ( B ) Fluorescence images of epithelium in branch-implanted lung explants treated with RepSox or vehicle control. The distance between implanted and native branches is small in RepSox-treated lungs. Scale bars, 100 µm and 10 µm (inset). ( C ) Fluorescence images of nuclei in lung explants cultured for 12- or 24-hours after implantation of beads with different TGFβ concentrations. ( D ) Graph showing the sizes of condensations in bead-implanted lung explants with different initial TGFβ concentration and culture time. ( E ) Fluorescence images of nuclei (blue) and pSMAD2 (red) counterstained for E-cadherin (green). Strong circular pSMAD2 staining is observed near TGFβ-containing beads. ( F ) Fluorescence images of nuclei (blue) and perlecan (red) in lung explants inserted with TGFβ-containing bead or vehicle control. Strong perlecan staining is observed in mesenchymal condensations near TGFβ-containing beads. ( G ) Fluorescence images of nuclei in lung explants cultured with or without RepSox after being inserted with TGFβ-containing beads or vehicle control. Mesenchymal condensations are only observed in lungs inserted with TGFβ-containing beads and cultured without RepSox. ( H ) pSMAD2 staining and nuclear-masked pSMAD2 staining intensity near the branch. Scale bar, 50 µm. Subepithelial mesenchymal cells exhibit strong pSMAD2 staining. Cyan dotted lines denote condensations ( D, F, G ). Scale bars, 100 µm ( A, C, E, F, G ).

Techniques: Fluorescence, Control, Cell Culture, Concentration Assay, Staining

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rabbit monoclonal anti psmad2 (Cell Signaling Technology Inc)

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